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What ROX concentration should I use in my qPCR reaction?

Instrument Compatibility & Recommendations:

Based on instrument manufacturers’ recommendations, we recommend the following compabilities:

 

High concentration ROX
Analytica Jena: qTower
Applied Biosystems: 7000, 7300, 7700, 7900, 7900HT, 7900HT FAST, StepOne™, StepOnePlus™
Cepheid: Smartcycler®
Eppendorf: Mastercycler® ep REALPLEX®, Mastercycler REALPLEX 2S
Illumina: Eco™
QIAGEN: Rotor-Gene® 3000, 6000, Q
Roche Applied Science: Lightcycler 480, Lightcycler Nano
Takara: Cycler Dice™
Techne: Quantica®

Low concentration ROX
Analytica Jena: qTower
Applied Biosystems: 7500, 7500 FAST, Viia™ 7
Bio-Rad: iCycler®, MyiQ™, iQ™ 5, Opticon™, Opticon 2, Chromo4™, MiniOpticon™ , CFX96™, CFX384™
Cepheid: Smartcycler®
Eppendorf: Mastercycler® ep REALPLEX®, Mastercycler REALPLEX 2S Eco™
Illumina: Eco™
QIAGEN: Rotor-Gene® 3000, 6000, Q
Roche Applied Science: Lightcycler® 480, Lightcycler Nano
Agilent: Mx4000P®, Mx3000P®, Mx3005P®
Takara: Cycler Dice™
Techne: Quantica®

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