Cytosine Methylation is along with Histone modification and ncRNAs the main epigenetic mechanism of the cells. These marks can increase or decrease Expression in different conditions.
The study of Methylation requires a Bisulfite Pyrosenquencing Procedure because is the only technique that can detect Cytosine Methylation at this level nowadays.
A knowledge of the different Methylation Patterns under different conditions can clarify how the ambient can influence the cell functionality.
Workflow for Methylation Analysis:
- Quality check of data.
- Identification of Methylated and unmethylated Cytosines.
- Differential Methylation Analysis.
- Comprehensive Report.
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