One of the most popular and useful procedures in Bioinformatics, Mapping, is used as basic step in larger analysis. It consists in the positioning of sequences over a larger sequence, typically a Genome.
We align the RNA-Seq reads against this Genome to Assemble the reads into transcripts.
Its applications are mainly re-sequencing (of RNA), variant analysis, alternative splicing study or localization of sequences among other.
Workflow for Reference-Guided Genome Assembly:
- Quality check of raw reads.
- Trimming of Adapters.
- Mapping against a Reference Genome.
- Comprehensive Report.
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