A Sequence Assembly consists on fitting together all the reads from a High-Throughput Sequencing to generate a set of contigs (large fragments separated by gaps) that make up a genome.
When there is a lack of reference genome, it is necessary to perform a de Novo assembly. This method is based on De Bruijn graphs. The reads are fragmented into k-mers and overlapped to build the genome with a minimum number of gaps.
Workflow for Whole Genome De Novo Assembly:
- Quality check of raw reads.
- Trimming of Adapters.
- Comprehensive Report.
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