ELISA & Immunoassay Development

Canvax has extensive experience in the transfer, development, and validation of a wide-range of ELISA and other plate-based assay configurations.

1-Immunoassay Definition

• Immunoassay: Sandwich assay, competitive, indirect, qualitative or quantitative, etc.
• Signal transduction: chromogenic, fluorogenic, etc.

2- Raw materials and Protocol Definition:

• Microplate: chemical surface, brand, format.
• Concentration and type of capture molecule: antigen, mAb, aptamer,etc) and detection molecule (antigen, antibody).
• Detection molecule conjugation with different labels: enzyme, fluorochrome, etc.
• Buffer composition and pH of the different reagents: blocking buffer, sample diluent, conjugate diluent, washing buffer, stop solution, etc.
• Type of substrate.
• Time and temperature of incubation.

3- Final Product Characterization

• Inter and intra: (lot, assay, lab and user) reproducibility.
• Limit of Detection, low and high limits of quantification, linear range.
• Sample analysis, diagnostic and analytical specificity and sensibility.
• Stability Studies.

4- Immunoassay Production

• Pilot production and scale-up manufacturing.
• Production of ELISA immunoassays designed externally.

Advantages & Features

• Highly Specialized & Largest Range: 300+ different Life Sciences Services availables.
• Latest Equipment & Technologies: made in Canvax™ Headquarters, based in Córdoba (Spain), a 1,500 m² fully-equipped lab.
• Experienced Staff: Canvax™ PhD Team has an average 20+ years’ experience hands-on.
• Cost-Advantage: due the use of Highly Innovative & Premium Quality Products, manufactured by Canvax™.
• Trustworthy: Confidentiality Agreement, Technical Advice, regular updates, Go-No Go steps, and much more.
• Custom-tailored & Modularized Services: to assure that every step meets your needs.
• Bilingual Technical Support: for easier understanding, your inquiries would be answered in Spanish or English.